商品編號:P0144600307002 原始貨號:K-LATE
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L-乳酸檢測套組 L-Lactic Acid (L-Lactate) Assay Kit

Megazyme台灣區正式授權總代理
產品編號: No.  700004310
目錄編號: No.  K-LATE
  • 用於測量樣品中L-乳酸含量
  • 此套組樣品來源適用於酒類、軟性飲料、牛奶、乳製品、醋、烘培食品、蛋製品、糖果、點心、冰淇淋、蔬果、加工蔬果、肉製品、食品添加物、化妝品、調味品和其他材料(例如:生物培養材料)...等
  • 檢測原理:
    1️⃣乳酸含量➡️樣品中的L-乳酸經酵素( L- lactate dehydrogenase)作用後會產生丙酮酸(Pyruvate)加上NADH。之後丙酮酸在D-glutamate存在的情況下,會被酵素(D-glutamate-pyruvate transaminase)作用,其產物為D-Alanine + 2-Oxoglutarate。分光光度計(spectrophotometer)在340 nm的波長下,可檢測NADH產生之吸光值(NAD+ 不會產生吸光值),後續再與標準溶液、空白對照組之吸光值前後對照,即可得到測試結果。
     
  • 此套組之測量結果,需搭配分光光度計(spectrophotometer)、微量多孔盤分析儀(Microplate)、自動分析儀(Auto-analyser)使用
  • 偵測極限: 0.21 mg/L
  • 線性範圍: 0.3 to 30 µg of L-lactic acid per assay
  • 便捷、易用、易學、易上手
  • 節省設備建置、實驗室空間、耗材、委外送驗等開銷
  • 提供線上可下載之計算工具,原始數據輕鬆處理
  • 總測試時間: 約10分鐘
  • 應用:食品研發、學術研究、原物料和成品檢驗
  • 冷藏(2–8°C)可短期存放,如需長時間保存,請參考瓶身的保存方式(酵素保存方式皆不相同)
     
  • 供應規格:
    50 assays (manual) / 500 assays (microplate) / 450 assays (auto-analyser)
  • 供應規格:此套組內僅提供酵素溶液,其他藥品及耗材需另購
     
  • 國際認證:
    基於此原理之檢測方法,已被DIN, GOST, IDF, EEC, EN, ISO, OIV, IFU, AIJN and MEBAK 所接受
     
  • 此商品交期約30-45天,可接受在下單。
  •     7天鑑賞期後即可折抵
數量
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  • 原價 : $ 9,999,999

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  • A.  MANUAL ASSAY PROCEDURE:



     
  • B.  AUTO-ANALYSER ASSAY PROCEDURE:



     
  • C.  MICROPLATE ASSAY PROCEDURE:



     
  • SAMPLE PREPARATION:

    1.  Sample dilution.
    The amount of L-lactic acid present in the cuvette (i.e. in the 0.1 mL of sample being analysed) should range between 0.3 and 30 μg.  The sample solution must therefore be diluted sufficiently to yield an L-lactic acid concentration between 0.003 and 0.30 g/L.


    2.  Sample clarification.

    a.  Solutions:
    Carrez I solution.  Dissolve 3.60 g of potassium hexacyanoferrate (II) {K4[Fe(CN)6].3H2O} (Sigma cat. no. P9387) in 100 mL of distilled water.  Store at room temperature.
    Carrez II solution.  Dissolve 7.20 g of zinc sulphate (ZnSO4.7H2O)  (Sigma cat. no. Z4750) in 100 mL of distilled water.  Store at room temperature.
    Sodium hydroxide (NaOH, 100 mM).  Dissolve 4 g of NaOH in 1 L of distilled water.  Store at room temperature.

    b.  Procedure:
    Pipette the liquid sample into a 100 mL volumetric flask which contains approx. 60 mL of distilled water, or weigh sufficient quantity of the sample into a 100 mL volumetric flask and add 60 mL of distilled water.  Carefully add 5 mL of Carrez I solution, 5 mL of Carrez II solution and 10 mL of NaOH solution (100 mM).  Mix after each addition.  Fill the volumetric flask to the mark, mix and filter.

    3.  General considerations.
    (a)  Liquid samples: for clear, slightly coloured liquid samples, adjust the pH to approx. 10 and use directly in the assay.
    (b)  Acidic samples: if > 0.1 mL of an acidic sample is to be used undiluted (such as wine or fruit juice), the pH of the solution should be increased to approx. 10.0 using 2 M NaOH, and the solution incubated at room temperature for 30 min.
    (c)  Carbon dioxide: samples containing a significant amount of carbon dioxide, such as beer, should be degassed by increasing the pH to approx. 10.0 with 2 M NaOH and gentle stirring, or by stirring with a glass rod.
    (d)  Coloured samples: an additional sample blank, i.e. sample with no L-LDH, may be necessary in the case of coloured samples.
    (e)  Strongly coloured samples: if used undiluted, strongly coloured samples should be treated by the addition of 0.2 g of polyvinylpolypyrrolidone (PVPP) per 10 mL of sample.  Shake the tube vigorously for 5 min and then filter through Whatman No. 1 filter paper.
    (f)  Solid samples: homogenise or crush solid samples in distilled water and filter if necessary.
    (g)  Samples containing fat: extract such samples with hot water at a temperature above the melting point of the fat, e.g. in a 100 mL volumetric flask at 60°C.  Allow to cool to room temperature and fill the volumetric flask to the mark with distilled water.  Store on ice or in a refrigerator for 15-30 min to allow the fat to separate and then filter.  Discard the first few mL of filtrate and use the clear supernatant (which may be slightly opalescent) for assay.  Alternatively, clarify with Carrez reagents.
    (h)  Samples containing protein: deproteinise samples containing protein by adding an equal volume of ice-cold 1 M perchloric acid with mixing.  Centrifuge at 1,500 g for 10 min and neutralise the supernatant with 1 M KOH.  Alternatively, use Carrez reagents.

商品規格

  • 商品規格    
    50 assays (manual) / 500 assays (microplate) / 450 assays (auto-analyser)

  • Bottle 1:  
    Buffer (25 mL, pH 10.0) plus D-glutamate and sodium azide (0.02% w/v) as a preservative.
    Stable for > 2 years at 4°C.

    Bottle 2: 
    NAD+/PVP. 
    Stable for > 5 years below -10°C.
     
    Bottle 3: 
    D-Glutamate-pyruvate transaminase suspension (1.1 mL). 
    Stable for > 2 years at 4°C.

    Bottle 4: 
    L-Lactate dehydrogenase suspension (1.1 mL).
    Stable for > 2 years at 4°C.

    Bottle 5: 
    L-Lactic acid standard solution (5 mL, 0.15 mg/mL)  in 0.02% (w/v) sodium azide.
    Stable for > 2 years at 4°C.

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