Zearalenone 玉米赤黴烯酮-定量-快篩試紙-水溶性萃取

• 原理
  側流體免疫層析法 (lateral flow immunochromatographic assay)，為快篩試劑的使用原理，藉由抗體抗原間的專一性與免疫親和力做檢測，被普遍應用在、病毒感染確認、妊娠測試、警局臨檢驗毒等方面。此方法多用於定性檢測，且顯色標定物上也有許多不同的種類，目前大多數的顯色方式是利用抗體吸附在膠體金（colloid gold）來呈色。
  
  玉米赤黴烯酮(Zearalenone)又稱玉米烯酮或F-2 toxin，此毒素主要由真菌-鐮孢菌（Fusarium spp.）產生，玉米赤黴烯酮由於在結構上類似雌激素，因此能與雌激素受體結合，引發類似雌激素之效應，故此毒素主要針對動物之生殖系統產生毒性
   
• 結果判讀
  玉米極限:
  偵測極限(limit of detection, LOD)： 36 ppb 
  定量極限(limit of quantification, LOQ)：50 ppb
  
  小麥極限:
  偵測極限(limit of detection, LOD)： 21 ppb 
  定量極限(limit of quantification, LOQ)：25 ppb
  
  偵測範圍:25-500 ppb(樣品超過500ppb需重新稀釋及測量)
   
• 樣品準備(SAMPLE PREPARATION)
  The sample to be tested should be collected according to accepted sampling techniques (see FGIS sampling protocol or contact your Neogen representative). Obtain a representative sample (minimum 100 g). Grind the sample so at least 95% of the ground material passes through a 20 mesh sieve (about the particle size of fine espresso).
   
• 樣品萃取(SAMPLE EXTRACTION)
  1. Weigh out 10 ± 0.1 g of sample into extraction cup.
  2. Add contents of one (1) MAX 1 aqueous extraction packet to the extraction cup.
  3. Add 50 mL distilled or deionized water to the extraction cup.
  4. Vigorously shake, using hand or mechanical means, for 3 minutes, or blend for 1 minute.
  5. Allow the sample to settle, then filter with a filter syringe or Whatman No. 4 filter paper, collecting a minimum of 3 mL filtrate into a sample collection tube.
   
• 玉米和小麥樣品操作流程(TEST PROCEDURE)
  1. Place the appropriate number of red sample dilution cups into a sample cup rack. Label cups if necessary.
  2. Add 250 µL of sample diluent (red-labeled bottle) to each red sample dilution cup.
  3. Add 250 µL of sample extract to the red sample dilution cup containing the sample diluent. Mix by pipetting up and down 5 times. Continue to step 4 below.
   
• 玉米筋粉樣品操作流程(TEST PROCEDURE)(Corn gluten meal)
  1. Place the appropriate number of red sample dilution cups and clear sample cups into a sample cup rack. Label cups if necessary.
  2. Add 200 µL of sample diluent (red-labeled bottle) to each red sample dilution cup.
  3. Add 300 µL of sample extract to the red sample dilution cup containing the sample diluent. Mix by pipetting up and down 5 times. Continue to step 4 below.
  4. Fully insert a Reveal Q+ MAX for Zearalenone test strip into a Raptor cartridge.
  5. Insert the Raptor cartridge containing the test strip into any of the three ports within the Raptor.
  a. The bar code on the test strip will be read. The system identifies the type of test strip and the lot number. If the lot number is not found in the system, the bar code reader on the front of the Raptor will turn on automatically.
  b. Scan the QR code found on the tube containing the test strips. The information will be stored on the reader. (The QR code can also be found on the kit Certificate of Analysis.)
  6. Enter the Sample ID if desired.
  7. Select Curve 1 for wheat or Curve 2 for corn and corn gluten meal samples.
  8. Add 400 µL of sample from the red sample dilution cup to the Raptor cartridge.
  a. The Raptor system will start automatically.
  b. Additional samples can be started in the other ports while the first sample is processing.
  9. Results will be displayed on the Raptor screen after the 5-minute incubation is complete.
   
• 小麥和玉米樣品稀釋步驟(Dilution Procedure) (樣品超過500ppb需重新稀釋及測量)
  1. Add 200 µL of sample filtrate to a sample collection tube.
  2. Add 400 µL of dilution buffer (yellow-labeled bottle) to the sample collection tube. Mix by pipetting up and down 5 times.
  3. Add 250 µL of sample diluent (red-labeled bottle) to a red sample dilution cup.
  4. Add 250 µL of sample extract to the red sample dilution cup containing the sample diluent. Mix by pipetting up and down 5 times. Continue to step 5 in the following section.
   
• 玉米筋粉樣品稀釋步驟(Dilution Procedure)(Corn gluten meal) (樣品超過500ppb需重新稀釋及測量)
  1. Add 200 µL of sample filtrate to a sample collection tube.
  2. Add 400 µL dilution buffer (yellow-labeled bottle) to the sample collection tube. Mix by pipetting up and down 5 times.
  3. Add 200 µL of sample diluent (red-labeled bottle) to a red sample dilution cup.
  4. Add 300 µL of sample extract to the red sample dilution cup containing the sample diluent. Mix by pipetting up and down 5 times. Continue to step 5 below.
  5. Fully insert a Reveal Q+ MAX for Zearalenone test strip into a Raptor cartridge.
  6. Insert the Raptor cartridge containing the test strip into any of the three ports within the Raptor.
  a. The bar code on the test strip will be read.  The system identifies the type of test strip and the lot number. If the lot number is not found in the system, the bar code reader on the front of the Raptor will turn on automatically.
  b. Scan the QR code found on the tube containing the test strips. The information will be stored on the reader. (The QR code can also be found on the kit Certificate of Analysis.)
  7. Enter the Sample ID if desired.
  8. Select Curve 1 for wheat or Curve 2 for corn and corn gluten meal samples.
  9. Add 400 µL of sample from the red sample dilution cup to the Raptor cartridge.
  10. The Raptor system will start automatically.
  11. Additional samples can be started in the other ports while the first sample is processing.
  12. Results will be displayed on the Raptor screen after the 5- minute incubation is complete.
  13. Multiply your result by 3.

• 產品說明書：連結
• 操作手冊：連結
• 物質安全資料表(Safety Data Sheet; SDS)：中文連結、英文連結

參考規範：食品中污染物質及毒素衛生標準