Vomitoxin嘔吐毒素(DON)-定量-快篩試紙- MAX 1水溶性萃取-偵測儀器No. 9680

• 原理
  側流體免疫層析法 (lateral flow immunochromatographic assay)，為快篩試劑的使用原理，藉由抗體抗原間的專一性與免疫親和力做檢測，被普遍應用在、病毒感染確認、妊娠測試、警局臨檢驗毒等方面。此方法多用於定性檢測，且顯色標定物上也有許多不同的種類，目前大多數的顯色方式是利用抗體吸附在膠體金（colloid gold）來呈色。
  
  Vomitoxin嘔吐毒素，又稱脫氧雪腐鐮刀菌烯醇(deoxynivalenol, 簡稱DON)，屬於鐮刀黴菌毒素(新月毒素群)(trichothecenes)的一種，主要是由禾穀鐮孢菌(Fusarium graminearum)汙染大麥、小麥、玉米及相關的穀物飼料加工品所產生的天然毒素，DON最明顯的症狀是引起動物的嘔吐，此外，也會引起其他急性症狀，例如:腹痛、腹瀉、腸胃炎...等。
• 結果判讀
  極限:
  偵測極限(limit of detection, LOD)： 0.3 ppm 
  定量極限(limit of quantification, LOQ)：0.3 ppm
  偵測範圍:0.3–6 ppm(樣品超過6ppm需重新稀釋及測量)
   
• 樣品準備(Sample Preperation)
  The sample to be tested should be collected according to accepted sampling techniques (contact your NEOGEN representative). Obtain a representative sample (minimum 100 g). Grind the sample so at least 95% of the ground material passes through a 20 mesh sieve (about the particle size of fine espresso).
   
• 樣品萃取(SAMPLE EXTRACTION)
  1. Weigh out 10 ± 0.1 g of sample into extraction cup.
  2. Add contents of 1 MAX 1 aqueous extraction packet to the extraction cup.
  3. Add 50 mL distilled or deionized water to the extraction cup.
  4. Vigorously shake, using hand or mechanical means, for 3 minutes, or blend.
  5. Allow the sample to settle, then filter with a filter syringe or Whatman No. 4 filter paper.
  6. You may also pipette 1 mL of sample into a micro-centrifuge tube, and centrifuge for 30 seconds using a micro-centrifuge.
  7.  The sample is now ready for testing.
   
• 樣品萃取(SAMPLE EXTRACTION)— 50 g (FGIS Method) 
  1. Weigh out 50 g ± 0.1 g of sample into extraction container.
  2. Add contents of 1 MAX 1-G50 aqueous extraction packet to the extraction cup.
  3. Add 250 mL distilled or deionized water to the extraction container.
  4. Blend for 30 seconds.
  5. Pipette sample into a 2.0 mL micro-centrifuge tube, and centrifuge for 30 seconds at 6000 RPM using a micro-centrifuge.
   
• 操作流程(TEST PROCEDURE)— Raptor Integrated Analysis Platform
  1. Place the appropriate number of red sample dilution cups into a sample cup rack. Label cups if necessary.
  2. Add 100 µL of sample extract to each red sample dilution cup.
  3. Add 1,000 µL of sample diluent to each red sample dilution cup. Mix by pipetting up and down 5 times.
  4. Fully insert a Reveal Q+ MAX for DON test strip into a Raptor cartridge.
  5. Insert the Raptor cartridge containing the test strip into any of the 3 ports within the Raptor Integrated Analysis Platform reader. 
  a. The barcode on the test strip will be read — the Raptor reader identifies the type   of test strip and the lot number. If the lot number is not found in the system, the   barcode reader in the front of the Raptor will turn on automatically.
  b. Scan the QR code found on the tube containing the test strips. The information will   be stored on the system.
  6. Enter sample ID if desired.
  7. Add 400 µL of sample extract from the red sample dilution cup to the Raptor cartridge.
  a. The Raptor reader will start automatically.
  b. Additional samples can be started in the other ports while the first sample is processing.
  8. Results will be displayed on the Raptor screen after the 3 minute testing is complete.
   
• 稀釋步驟(Dilution Procedure) — Raptor Integrated Analysis Platform(樣品超過6ppm需重新稀釋及測量)
  Samples greater than 6 ppm will need to be diluted and retested.
  1. Add 100 µL sample filtrate to a sample collection tube.
  2. Add 500 µL distilled or deionized water to the sample collection tube. Mix well by pipetting up and down 5 times, or cap the tube and shake.
  3. Add 100 µL of diluted sample extract (from step 2) to each red sample dilution cup.
  4. Add 1,000 µL of sample diluent (blue labeled bottle) to the red dilution cup with sample extract. Mix by pipetting up and down 5 times.
  5. Fully insert a Reveal Q+ MAX for DON test strip into a Raptor cartridge.
  6. Insert the Raptor cartridge containing the test strip into any of the 3 ports within the Raptor reader. 
  a.  The barcode on the test strip will be read — the Raptor reader identifies the type of test strip and the lot number. If the lot number is not found in the system, the barcode reader in the front of the Raptor will turn on automatically.
  b. Scan the QR code found on the tube containing the test strips. The information will be stored on the system.
  7. Enter sample ID if desired.
  8. Add 400 µL of sample extract from the red sample dilution cup to the Raptor cartridge.
  a. The Raptor reader will start automatically.
  b. Additional samples can be started in the other ports while the first sample is processing.
  9. Results will be displayed on the Raptor reader’s screen after the 3 minute testing is complete. 
  Note: The Raptor reader will not calculate your dilution. Final result displayed will need to be multiplied by 6

• 產品說明書：連結
• 操作手冊：連結
• 物質安全資料表(Safety Data Sheet; SDS)：中文連結、英文連結

參考規範：食品中污染物質及毒素衛生標準