AFLATOXIN 總黃麴毒素-定量-快篩試紙-水溶性萃取

• 原理
  側流體免疫層析法 (lateral flow immunochromatographic assay)，為快篩試劑的使用原理，藉由抗體抗原間的專一性與免疫親和力做檢測，被普遍應用在、病毒感染確認、妊娠測試、警局臨檢驗毒等方面。此方法多用於定性檢測，且顯色標定物上也有許多不同的種類，目前大多數的顯色方式是利用抗體吸附在膠體金（colloid gold）來呈色。
  
   
• 結果判讀
  極限:
  偵測極限(limit of detection, LOD)： 2 ppb 
  定量極限(limit of quantification, LOQ)：3 ppb
  偵測範圍:3–300 ppb(樣品超過50ppb需重新稀釋及測量)
   
• 樣品前置處理(SAMPLE PREPARATION)
  The sample to be tested should be collected according to accepted sampling techniques (see FGIS sampling protocol or contact your Neogen representative). Obtain a representative sample (minimum 100 gm). Grind the sample so at least 95% of the ground material passes through a 20 mesh sieve (about the particle size of fine espresso).
   
• 樣品萃取(SAMPLE EXTRACTION)
  1. Weigh out 10 g ± 0.1 g of sample into extraction cup.
  2. Add contents of one (1) MAX 1 aqueous extraction packet to the extraction cup.
  3. Add 50 mL distilled or deionized water to the extraction cup.
  4. Vigorously shake, using hand or mechanical means, for 3 minutes, or blend for 1 minute.
  5. Allow the sample to settle, then filter with a filter syringe or Whatman #4 filter pa per to collect a minimum of 3 mL filtrate into a sample collection tube. Or, you may also pipette 1 mL of sample into a 2.0 mL microcentrifuge tube, and centrifuge for 1 minute using a microcentrifuge (approx. 2,000 x g). 
   
• 樣品萃取(SAMPLE EXTRACTION)(FGIS METHOD)
  1. Weigh out 50 g ± 0.1 g of sample into extraction container.
  2. Add contents of one (1) MAX 1-G50 aqueous extraction packet to the extraction cup.
  3. Add 250 mL distilled or deionized water to the extraction container.
  4. Vigorously shake, using hand or mechanical means, for 3 minutes.
  5. Allow the sample to settle, then filter with a filter syringe or Whatman #4 filter paper to collect a minimum of 3 mL filtrate into a sample collection tube. Or, you may also pipette 1 mL of sample into a 2.0 mL micro-centrifuge tube, and centrifuge for 1 minute using a micro-centrifuge (approx. 2,000 x g).
   
• 操作流程(TEST PROCEDURE)
  1. Place the appropriate number of red sample dilution cups into a sample cup rack. Label cups if necessary.
  2. Add 250 µL of sample extract to each red sample dilution cup.
  3. Add 250 µL of sample diluent to red sample dilution cup. Mix by pipetting up and down five times.
  4. Fully insert a Reveal Q+ MAX for Aflatoxin test strip into a Raptor cartridge.
  5. Insert the Raptor cartridge containing the test strip into any of the three ports within the Raptor.
  a. The bar code on the test strip will be read. The system identifies the type of test strip and the lot number. If the lot number is not found in the system, the bar code reader in the front of the Raptor will turn on automatically.
  b. Scan the QR code found on the tube containing the test strips. The information will be stored on the reader.
  6. Enter Sample ID if desired.
  7. Add 400 µL of sample extract from the red sample dilution cup to the Raptor cartridge. 
  a. The Raptor system will start automatically.
  b. Additional samples can be started in the other ports while the first sample is processing.
  8. Results will be displayed on the Raptor screen after the 6-minute testing period is complete.
   
• 稀釋步驟(樣品超過50ppb需重新稀釋及測量)
  1. Add 100 µL sample filtrate to a sample collection tube.
  2. Add 500 µL distilled or deionized water to the sample collection tube. Mix well by pipetting up and down 5 times, or cap the tube and shake.
  3. Add 250 µL of diluted sample extract (from step 2) to each red sample dilution cup.
  4. Add 250 µL of sample diluent (blue labeled bottle) to the red dilution cup with sample extract. Mix by pipetting up and down five times.
  5. Insert the Raptor cartridge containing the test strip into any of the three ports within the Raptor.
  a. The bar code on the test strip will be read. The system identifies the type of test strip and the lot number. If the lot number is not found in the system, the bar code reader in the front of the Raptor will turn on automatically. 
  b. Scan the QR code found on the tube containing the test strips. The information will be stored on the reader.
  6. Enter Sample ID if desired.
  7. Add 400 µL of sample extract from the red sample dilution cup to the Raptor cartridge. 
  a. The Raptor system will start automatically.
  b. Additional samples can be started in the other ports while the first sample is processing.
  8. Results will be displayed on the Raptor screen after the 6-minute testing period is complete.
  NOTE: The Raptor reader will not calculate your dilution. Final result displayed will need to be multiplied by 6.

• 產品說明書：連結
• 操作手冊：連結
• 物質安全資料表(Safety Data Sheet; SDS)：中文連結、英文連結

參考規範：食品中污染物質及毒素衛生標準

關於我國最新的＂食品中污染物質及毒素衛生標準＂完整版，請參照衛福部食藥署或全國法規資料庫。