- 成分
| Xylose | 3.5 g/L |
| L-Lysine | 5.0 g/L |
| Lactose Monohydrate | 7.5 g/L |
| Sucrose | 7.5 g/L |
| Sodium Chloride | 5.0 g/L |
| Yeast Extract | 3.0 g/L |
| Phenol Red | 0.08 g/L |
| Agar | 13.5 g/L |
| Sodium Deoxycholate | 2.5 g/L |
| Sodium Thiosulfate | 6.8 g/L |
| Ferric Ammonium Citrate | 0.8 g/L |
Final pH: 7.4 ± 0.2 at 25°C
The formula may be adjusted and/or supplemented as required to meet performance specifications.
- 配製方法
1. Suspend 55 grams of the medium in one liter of purified water.
2. Heat with frequent agitation until the medium reaches the boiling point. Once boiling has been reached remove from the heat immediately.
3. AVOID OVERHEATING. DO NOT AUTOCLAVE
<攪拌加熱至沸騰溶解即可，注意不可加熱過度>
4. Cool to 45-50°C.
5. Pour into plates as soon as the medium has cooled.
6. Protracted boiling or prolonged holding at elevated temperature induces precipitation.
Note: It is advisable to not prepare large volumes which will require prolonged heating.
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