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成分
| Columbia Blood Agar Base | 39.0 g/L |
| Esculin | 1.0 g/L |
| Ferric Ammonium Citrate | 0.5 g/L |
| Lithium Chloride | 15.0 g/L |
| Agar | 2.0 g/L |
Final pH: 7.0 ± 0.2 at 25°C
The formula may be adjusted and/or supplemented as required to meet performance specifications.
- 配製方法
Oxford Listeria Agar(OXA培養基)
1. Suspend 57.5 g of the medium in one liter of purified water.
2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
3. Autoclave at 121°C for 15 minutes.
4. Cool to 45 - 50°C.
5. Aseptically add 2 vials of NCM4047-0.5* Oxford Supplement (with Natamycin), each reconstituted
using 5 mL sterile 50% ethanol, or NCM4070-0.5* Oxford Supplement (with Cycloheximide), each
reconstituted using 5 mL sterile 50% ethanol and mix well before dispensing.
*Larger vials may be available. Please see appropriate supplement data sheet for availability and
preparation instructions.
Modified Oxford Listeria Agar(MOX培養基)
Perform steps 1-4 above and aseptically add 2 vials of NCM4080-0.5* Modified Oxford Agar Supplement,
each reconstituted using 5mL sterile deionized/RO water.
*Larger vials may be available. Please see appropriate supplement data sheet for availability and
preparation instructions.
- 根據上述食品中單核球增多性李斯特菌檢驗方法，OXA培養基(每升)會在滅菌後，加入環己胺0.4 g、大腸菌素硫酸鹽0.02 g、吖啶黃素0.005 g、頭孢泰坦0.002 g及弗斯弗黴素0.01 g之無水乙醇/蒸餾水(1:1, v/v)溶液10 mL
- 補充劑
- [必備] 如要配製OXA培養基(每升):需加入2瓶 →
NCM4070-0.5* Oxford Supplement (with Cycloheximide)
- [必備] 如要配製OXA培養基(每升):需加入2瓶 →
- [必備] 如要配製MOX培養基(每升):需加入2瓶 → NCM4080-0.5* Modified Oxford Agar Supplement
成分為追加：1%腸黏菌素溶液(1% colistin solution) 1mL，且滅菌後會加入拉他頭孢(sodium moxalactam)溶液2 mL
