- 成分
| Pancreatic Digest of Gelatin | 20.0 g/L |
| Magnesium Chloride | 1.4 g/L |
| Dipotassium Sulfate | 10.0 g/L |
| Cetrimide | 0.3 g/L |
| Agar | 13.6 g/L |
Final pH: 7.2 ± 0.2 at 25°C
The formula may be adjusted and/or supplemented as required to meet performance specifications.
- 配製方法
1. Suspend 45.3 grams of the medium in 1 liter of purified water.
2. Add 10mL of Glycerol.
3. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
4. Autoclave at 121°C for 15 minutes.
5. Cool to 45-50°C.
- 原理
- 溴化十六烷基三甲銨(C19H42BrN)：可抑制多數格蘭氏陽性桿菌和部分格蘭氏陰性桿菌
- 氯化鎂(MgCl2)+硫酸鉀(K2SO4)：可促進綠膿桿菌產生色素和毒素，進而在培養基上產生綠色菌落
