- 成分
| Enzymatic Digest of Casein | 15.0 g/L |
| Enzymatic Digest of Soya | 5.0 g/L |
| Yeast Extract | 5.0 g/L |
| Sodium Metabisulfite | 1.0 g/L |
| Ferric Ammonium Citrate | 1.0 g/L |
| 1Agar | 14.0 g/L |
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Final pH: 7.6 ± 0.2 at 25°C
The formula may be adjusted and/or supplemented as required to meet performance specifications.
備註1:衛福部公告之檢驗方法當中，TSC培養基之洋菜(agar)克數為20克
- 配製方法
1. Suspend 41 grams of the medium in 900 mL of purified water.
2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
3. Autoclave at 121°C for 15 minutes. Cool to 45 - 50°C.
4. Add 100 mL of NCM4017* Egg Yolk Emulsion, 50%, and the desired antimicrobic supplement:
TSC Agar: Add 2 vials of NCM4022-0.5* TSC Perfringens Supplement, each reconstituted using 5
mL sterile deionized/RO water.
*Larger vials may be available. Please see appropriate supplement data sheet for availability and
preparation instructions.
- Overlay Agar(雙層培養基用)
2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
3. Autoclave at 121°C for 15 minutes. Cool to 45 - 50°C.
4. Overlay agar does not get supplemented with Egg Yolk Emulsion. Overlay agar is held at 45 -50°C until used and should be poured right after inoculation.
