- 成分
| Letheen Agar Base | 25.0 g/L |
| Enzymatic Digest of Casein | 10.0 g/L |
| Enzymatic Digest of Animal Tissue | 10.0 g/L |
| Yeast Extract | 2.0 g/L |
| Sodium Chloride | 5.0 g/L |
| Sodium Bisulfite | 0.1 g/L |
Final pH: 7.2 ± 0.2 at 25°C
The formula may be adjusted and/or supplemented as required to meet performance specifications.
衛福部建議檢驗方法中，改良式Letheen培養基(Modified Letheen agar, MLA)成分如下:
| Letheen洋菜(Letheen agar) | 32 g |
| 胰化酪蛋白腖(trypticase peptone) | 5 g |
| 硫蛋白腖(thiotone peptone) | 10 g |
| 酵母抽出物(yeast extract) | 2 g |
| 氯化鈉 | 5 g |
| 亞硫酸氫鈉(NaHSO3) | 0.1 g |
| 洋菜(agar) | 5 g |
| 蒸餾水 | 1000 mL |
- 配製方法
1. Suspend 52.1 grams of the medium and 7 mL of NCM4081-100 Tween 80 in one liter of purified water.
2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
3. Autoclave at 121°C for 15 minutes.
4. Cool to 45-50°C.
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