- 成分
| Enzymatic Digest of Casein | 17.0 g/L |
| Enzymatic Digest of Soybean Meal | 3.0 g/L |
| Yeast Extract | 6.0 g/L |
| Dextrose | 2.5 g/L |
| Sodium Chloride | 5.0 g/L |
| Monopotassium Phosphate | 1.35 g/L |
| Dipotassium Phosphate | 2.5 g/L |
| Disodium Phosphate | 9.6 g/L |
Final pH: 7.3 ± 0.2 at 25°C
The formula may be adjusted and/or supplemented as required to meet performance specifications.
- 配製方法
1. Dissolve 47 grams of the medium in 1000 mL of purified water.
2. Mix thoroughly.
3. Autoclave at 121°C for 15 minutes.
4. Aseptically add 11.1 mL of a 10% filter sterilized solution of sodium pyruvate.
5. After four hours of incubation at 30 ± 2°C, aseptically add 2.5 mL NCM4068-0.5* Buffered Listeria
Enrichment Supplement (BAM) to 225 mL of Buffered Listeria Enrichment Broth Base. Buffered
Listeria Enrichment Supplement (BAM) should be reconstituted using 5 mL of sterile deionized/RO
water.
